Biosensing with SiO2-covered SPR substrates in a commercial SPR-tool
نویسندگان
چکیده
منابع مشابه
Advanced Methods for SPR Imaging Biosensing
Microarray biosensors have become an invaluable biotechnological tool for the rapid, multiplexed detection of surface bioaffinity interactions. For example, nucleic acid microarrays are currently being applied to the areas of genomics [1,2], genetic testing [3], gene expression [4,5], and single nucleotide polymorphism (SNP) genotyping [6,7]. Many researchers are also interested in developing p...
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The sensitivity and selectivity of surface plasmon resonance imaging (SPRI) biosensing with nucleic acid microarrays can be greatly enhanced by exploiting various nucleic acid ligases, nucleases, and polymerases that manipulate the surface-bound DNA and RNA. We describe here various examples from each of these different classes of surface enzyme chemistries that have been incorporated into nove...
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Multi-analyte detection of environmentally relevant pesticides is performed by using a two-channelled surface plasmon resonance (SPR) biosensor. The special design of the SPR instrument allows the determination of several analytes (DDT, chlorpyrifos and carbaryl) via different immobilization formats. First, simultaneous pesticide monitoring is possible by flowing chlorpyrifos, carbaryl or DDT s...
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The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) creates a comprehensive protein investigation approach wherein SPR is employed for protein quantification and MS is utilized to structurally characterize the proteins. In such, MS utterly complements the SPR detection and reveals intrinsic protein structural modifications that go unregistered via the SPR detection. Pr...
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Early and accurate disease diagnosis still remains a major challenge in clinical settings. Biomarkers could potentially provide useful tools for the detection and monitoring of disease progression, treatment safety and efficacy. Recent years have witnessed prodigious advancement in biosensor development with research directed towards rapid, real-time, label-free and sensitive biomarker detectio...
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ژورنال
عنوان ژورنال: Sensors and Actuators B: Chemical
سال: 2014
ISSN: 0925-4005
DOI: 10.1016/j.snb.2014.04.060